溴百里酚蓝是弱酸和弱碱的pH指示剂。染料和代谢产物。生物活性BromothymolBlue是一种pH指示剂，是测定弱酸弱碱时使用的一种指示剂，其酸性时为黄色，碱性时为蓝色。体外研究AfirstcharacterizationandcomparisonisdonebydevelopinganeasyanddirectmeasurementmethodbasedonapHindicatorsystemusingBromothymolBlue(BTB)astheindicatorandpotassiumphosphateasthebuffer.ApH-shiftassayisdevelopedonthebasisofBTBasthepHindicatorandpotassiumphosphateasthebuffercomponent.ThreepHindicatorsaretestedforthedirectdeterminationof2-2-keto-L-gulonicacid(2-KLG)productiononaplate.TheresultsshowthatBromothymolBlueissuperiortotheothertwoindicatorsintermsoftheobviouscolorchangeandasuitablepHrange(bluetoyellowatpH6.5-7.5).UpontheadditionofaBromothymolBluesolution(0.1%,w/v)toanagarplate,zonessurroundingcoloniesofK.vulgare07mutantschangetheircolorfrombluetoyellowbecauseK.vulgare07mutantsrelease2-KLGonagarplates,therebyacidifyingsurroundingareasaroundcolonies.用途用作酸碱指示剂用途酸碱指示剂,pH变色范围6.0(黄色)-7.6(蓝色)。吸附指示剂。